SGOT / AST Reagent Kit (IFCC)

Kinetic IFCC reagent for aspartate aminotransferase (AST, SGOT) activity in serum and plasma.

Method: IFCC kinetic (NADH decrease)
Sample: Serum free of haemolysis; heparin or EDTA plasma
Linear up to 300 U/L
Liquid stable, ready to use pack
Reads at 340 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of aspartate aminotransferase (SGOT, AST) activity in serum and plasma. Principle: AST catalyses the reaction between α-ketoglutarate and L-aspartate to give glutamate and oxaloacetate. With malate dehydrogenase (MDH), oxaloacetate reacts with NADH to give malate and NAD; the rate of NADH decrease at 340 nm is proportional to AST activity. If activity exceeds 300 U/L, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of aspartate aminotransferase (SGOT, AST) activity in serum and plasma.

Specifications

Specifications for SGOT / AST Reagent Kit (IFCC)
Shelf life	12 months
Storage	2–8 °C. Working reagent (4 volumes of reagent 1 to 1 volume of reagent 2) is stable for 30 days at 2–8 °C
Method	IFCC kinetic (NADH decrease)
Sample type	Serum free of haemolysis; heparin or EDTA plasma
Wavelength	340 nm
Reaction type	Kinetic (decreasing)
Reagent volume	1 mL
Sample volume	100 µL
Incubation	Lag 60 s, read 180 s, interval 60 s
Reaction temperature	37 °C
Light path	1 cm
Standard	Not applicable (factor 1746)
Linearity	300 U/L
Reagent composition	Reagent 1: enzyme reagent; reagent 2: substrate reagent
Reference interval	Male: 10–40 U/L; female: 9–32 U/L Reference intervals are indicative; each laboratory should establish its own (see the kit insert).
Blank	Distilled water