Albumin Reagent Kit (Bromocresol Green)

Bromocresol green reagent for quantitative albumin in serum.

Method: Bromocresol green (BCG)
Sample: Serum free of haemolysis
Linear up to 8 g/dL
Liquid stable, ready to use pack
Reads at 628 nm (600–650 nm)

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of albumin concentration in serum. Principle: At pH 4.2, albumin binds bromocresol green to give a blue-green complex; the absorbance change at 628 nm correlates with albumin concentration. If albumin exceeds 8 g/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of albumin concentration in serum.

Specifications

Specifications for Albumin Reagent Kit (Bromocresol Green)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Bromocresol green (BCG)
Sample type	Serum free of haemolysis
Wavelength	628 nm (600–650 nm)
Reaction type	End point
Reagent volume	1 mL
Sample volume	10 µL
Incubation	5 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	4 g/dL
Linearity	8 g/dL
Reagent composition	Reagent 1: BCG reagent; albumin standard 4 g/dL
Reference interval	3.8–5.4 g/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).