Calcium Reagent Kit (Arsenazo III)

Arsenazo III reagent for quantitative calcium in serum and urine.

Method: Arsenazo III
Sample: Serum free of haemolysis; urine diluted 1:3 with distilled water and adjusted to pH 3–4 with 0.1 N HCl
Linear up to 16 mg/dL
Liquid stable, ready to use pack
Reads at 650 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of calcium concentration in serum and urine. Principle: At neutral pH, calcium forms a complex with arsenazo III; the colour intensity is directly proportional to calcium concentration. If calcium exceeds 16 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of calcium concentration in serum and urine.

Specifications

Specifications for Calcium Reagent Kit (Arsenazo III)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Arsenazo III
Sample type	Serum free of haemolysis; urine diluted 1:3 with distilled water and adjusted to pH 3–4 with 0.1 N HCl
Wavelength	650 nm
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	25 µL
Incubation	5 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	10 mg/dL
Linearity	16 mg/dL
Reagent composition	Reagent 1: arsenazo III reagent; calcium standard 10 mg/dL
Reference interval	Serum: 8.8–10.2 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).