Albumin Reagent Kit (Bromocresol Green)
Bromocresol green reagent for quantitative albumin in serum.
Alkaline Phosphatase (ALP) Reagent Kit
Kinetic reagent for quantitative alkaline phosphatase activity in serum.
- Method: p-Nitrophenyl phosphate, kinetic
- Sample: Serum free of haemolysis
- Linear up to 1,600 U/L
- Liquid stable, ready to use pack
- Reads at 405 nm
For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.
Overview
For in vitro quantitative determination of alkaline phosphatase activity in serum. Principle: Alkaline phosphatase converts p-nitrophenyl phosphate to p-nitrophenol and phosphate; the absorbance increase at 405 nm is proportional to enzyme activity. If activity exceeds 1,600 U/L, dilute the sample, re-assay and multiply by the dilution factor.
Intended use: For in vitro quantitative determination of alkaline phosphatase activity in serum.
Specifications
| Shelf life | 12 months |
|---|---|
| Storage | 2–8 °C, protected from light |
| Method | p-Nitrophenyl phosphate, kinetic |
| Sample type | Serum free of haemolysis |
| Wavelength | 405 nm |
| Reaction type | Kinetic (increasing) |
| Reagent volume | 1 mL |
| Sample volume | 20 µL |
| Incubation | Lag 60 s, read 90 s, interval 30 s |
| Reaction temperature | 37 °C |
| Light path | 1 cm |
| Standard | Not applicable (factor 2720) |
| Linearity | 1,600 U/L |
| Reagent composition | Reagent 1: substrate reagent |
| Reference interval | Adults: 25–140 U/L Reference intervals are indicative; each laboratory should establish its own (see the kit insert). |
| Blank | Distilled water |
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