Amylase Reagent Kit (CNPG2)

Kinetic reagent for quantitative alpha-amylase activity in serum and urine.

Method: CNP-oligosaccharide substrate, kinetic
Sample: Serum free of haemolysis; urine collected in clean, dry containers and kept at 2–8 °C
Linear up to 1,500 U/L
Liquid stable, ready to use pack
Reads at 405 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of amylase activity in serum and urine. Principle: Alpha-amylase hydrolyses the substrate 2-chloro-4-nitrophenyl-α-galactosylmaltoside (GAL-G2-CNP); the rate of p-nitrophenyl formation, read at 405 nm, is proportional to amylase activity. Chelating agents interfere: do not use citrate, oxalate or EDTA anticoagulants.

Intended use: For in vitro quantitative determination of amylase activity in serum and urine.

Specifications

Specifications for Amylase Reagent Kit (CNPG2)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	CNP-oligosaccharide substrate, kinetic
Sample type	Serum free of haemolysis; urine collected in clean, dry containers and kept at 2–8 °C
Wavelength	405 nm
Reaction type	Kinetic
Reagent volume	1 mL
Sample volume	20 µL
Incubation	Lag 60 s, read 180 s, interval 60 s
Reaction temperature	37 °C
Light path	1 cm
Standard	Not applicable (factor 3178)
Linearity	1,500 U/L
Reagent composition	Reagent 1: amylase substrate reagent
Reference interval	Serum: up to 90 U/L; urine: up to 480 U/L Reference intervals are indicative; each laboratory should establish its own (see the kit insert).