Phosphorus Reagent Kit (Ammonium Molybdate)

Ammonium molybdate reagent for quantitative inorganic phosphorus in serum and urine.

Method: Ammonium molybdate
Sample: Serum free of haemolysis; urine diluted 1:10 with distilled water
Linear up to 10.0 mg/dL
Liquid stable, ready to use pack
Reads at 340 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of inorganic phosphorus in serum and urine. Principle: Inorganic phosphate reacts with ammonium molybdate in sulphuric acid to form a phosphomolybdic complex, read at 340 nm; no protein-free filtrate is needed. If phosphorus exceeds 10 mg/dL, dilute the sample and re-assay.

Intended use: For in vitro quantitative determination of inorganic phosphorus in serum and urine.

Specifications

Specifications for Phosphorus Reagent Kit (Ammonium Molybdate)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Ammonium molybdate
Sample type	Serum free of haemolysis; urine diluted 1:10 with distilled water
Wavelength	340 nm
Reaction type	End point
Reagent volume	1 mL
Sample volume	10 µL
Incubation	5 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	5.0 mg/dL
Linearity	10.0 mg/dL
Reagent composition	Reagent 1: phosphorus reagent; phosphorus standard 5 mg/dL
Reference interval	Adults: 2.8–4.5 mg/dL; children: 4.0–7.0 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).