Microprotein Reagent Kit (Pyrogallol Red)

Pyrogallol red dye-binding reagent for protein in urine and cerebrospinal fluid.

Method: Pyrogallol red-molybdate dye binding
Sample: Random or 24-hour urine; CSF free of haemolysis (centrifuge any specimen containing red cells or particles)
Linear up to 200 mg/dL
Liquid stable, ready to use pack
Reads at 600 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of protein in urine and cerebrospinal fluid (CSF). Principle: A pyrogallol red-molybdate complex, modified to equalise the reactivity of albumin and gamma-globulin, binds protein under acidic conditions to give a blue-purple colour, read at 600 nm. CSF can be kept at 2–8 °C for several days before the assay. If protein exceeds 200 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of protein in urine and cerebrospinal fluid (CSF).

Specifications

Specifications for Microprotein Reagent Kit (Pyrogallol Red)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Pyrogallol red-molybdate dye binding
Sample type	Random or 24-hour urine; CSF free of haemolysis (centrifuge any specimen containing red cells or particles)
Wavelength	600 nm
Reaction type	End point
Reagent volume	1 mL
Sample volume	20 µL
Incubation	10 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	100 mg/dL
Linearity	200 mg/dL
Reagent composition	Reagent 1: pyrogallol red reagent; CSF protein standard 100 mg/dL
Reference interval	CSF: 8–43 mg/dL; random urine: 1–14 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).