LDL Cholesterol Reagent Kit (Direct, Enzymatic)

Direct enzymatic reagent for LDL cholesterol in serum and plasma.

Method: Direct enzymatic
Sample: Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Linear up to 250 mg/dL
Liquid stable, ready to use pack
Reads at 546 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of low-density lipoprotein cholesterol (LDL-C) in human serum and plasma. Principle: A surfactant selectively solubilises LDL in a cholesterol assay system using cholesterol esterase and cholesterol oxidase; other lipoproteins are inhibited by the surfactant and sugar compounds. LDL cholesterol is therefore measured directly. If LDL-C exceeds 250 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of low-density lipoprotein cholesterol (LDL-C) in human serum and plasma.

Specifications

Specifications for LDL Cholesterol Reagent Kit (Direct, Enzymatic)
Shelf life	12 months
Storage	2–8 °C. Reagents 1 and 2 are ready to use and stable to the expiry date on the label
Method	Direct enzymatic
Sample type	Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Wavelength	546 nm
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	10 µL
Incubation	10 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	Calibrator (concentration printed on the vial)
Linearity	250 mg/dL
Reagent composition	Reagent 1: buffer; reagent 2: substrate; LDL-C calibrator
Reference interval	Optimal: below 100 mg/dL; high: 160 mg/dL and above Reference intervals are indicative; each laboratory should establish its own (see the kit insert).