HDL Cholesterol Reagent Kit (Direct, Enzymatic)

Direct enzymatic reagent for HDL cholesterol in serum and plasma, with no pre-treatment or centrifugation.

Method: Direct enzymatic
Sample: Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Linear up to 200 mg/dL
Liquid stable, ready to use pack
Reads at 546 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of high-density lipoprotein cholesterol (HDL-C) in serum and plasma. Principle: A detergent solubilises only HDL, so HDL cholesterol is released to react with cholesterol esterase, cholesterol oxidase and chromogens to give colour. The colour intensity is proportional to HDL cholesterol. No sample pre-treatment or centrifugation is needed. If HDL-C exceeds 200 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of high-density lipoprotein cholesterol (HDL-C) in serum and plasma.

Specifications

Specifications for HDL Cholesterol Reagent Kit (Direct, Enzymatic)
Shelf life	12 months
Storage	2–8 °C. Reagents 1 and 2 are ready to use and stable to the expiry date on the label
Method	Direct enzymatic
Sample type	Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Wavelength	546 nm
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	10 µL
Incubation	10 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	Calibrator (concentration printed on the vial)
Linearity	200 mg/dL
Reagent composition	Reagent 1: buffer; reagent 2: substrate; HDL-C calibrator
Reference interval	Adult male: 35–79.5 mg/dL; adult female: 42–88 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).