HDL Cholesterol Precipitating Reagent Kit (Phosphotungstic Acid)

Precipitating reagent for HDL cholesterol in serum and plasma, used with a total cholesterol reagent.

Method: Phosphotungstic acid precipitation
Sample: Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Liquid stable, ready to use
Liquid stable, ready to use pack
Reads at 505 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of high-density lipoprotein cholesterol (HDL-C) in serum and plasma, used together with a total cholesterol reagent. Principle: Low-density fractions (LDL, VLDL) are precipitated with phosphotungstic acid and magnesium chloride and removed by centrifugation. HDL cholesterol in the clear supernatant is measured with the same reagent used for total cholesterol.

Intended use: For in vitro quantitative determination of high-density lipoprotein cholesterol (HDL-C) in serum and plasma, used together with a total cholesterol reagent.

Specifications

Specifications for HDL Cholesterol Precipitating Reagent Kit (Phosphotungstic Acid)
Shelf life	12 months
Storage	2–8 °C
Method	Phosphotungstic acid precipitation
Sample type	Serum or heparinised plasma, free of haemolysis, separated from the clot as soon as possible
Wavelength	505 nm
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	10 µL
Incubation	5 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	Cholesterol standard 200 mg/dL
Reagent composition	Reagent 1: HDL cholesterol precipitating reagent; cholesterol standard 200 mg/dL
Reference interval	30–70 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).
Blank	Cholesterol reagent