Glucose Reagent Kit (GOD-POD)

Enzymatic colorimetric reagent for quantitative glucose in serum, plasma, urine and cerebrospinal fluid.

Method: Enzymatic colorimetric (GOD-POD, Trinder)
Sample: Serum free of haemolysis, plasma, urine, cerebrospinal fluid
Linear up to 500 mg/dL
Liquid stable, ready to use pack
Reads at 505 nm (492–520 nm)

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of glucose concentration in serum, plasma, urine and cerebrospinal fluid. Principle: Glucose oxidase (GOD) converts glucose to gluconate and hydrogen peroxide; peroxidase (POD) then reacts the peroxide with phenol and 4-aminoantipyrine to give a coloured product, measured at 505 nm. The absorbance increase is proportional to the glucose concentration. If glucose exceeds 500 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of glucose concentration in serum, plasma, urine and cerebrospinal fluid.

Specifications

Specifications for Glucose Reagent Kit (GOD-POD)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Enzymatic colorimetric (GOD-POD, Trinder)
Sample type	Serum free of haemolysis, plasma, urine, cerebrospinal fluid
Wavelength	505 nm (492–520 nm)
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	10 µL
Incubation	5 minutes
Reaction temperature	37 °C
Light path	1 cm
Standard	100 mg/dL
Linearity	500 mg/dL
Reagent composition	Reagent 1: enzyme reagent; glucose standard 100 mg/dL
Reference interval	70–100 mg/dL (fasting) Reference intervals are indicative; each laboratory should establish its own (see the kit insert).