Creatinine Reagent Kit (Jaffe Kinetic)

Alkaline picrate (Jaffe) kinetic reagent for quantitative creatinine in serum and urine.

Method: Jaffe kinetic (alkaline picrate)
Sample: Serum free of haemolysis; 12 h or 24 h urine diluted 1:100 with distilled water
Linear up to 25 mg/dL
Liquid stable, ready to use pack
Reads at 492 nm (480–520 nm)

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of creatinine in serum and urine. Principle: Creatinine forms a coloured creatinine-picrate complex with alkaline picrate (1:1); the rate of colour formation is proportional to creatinine concentration. If creatinine exceeds 25 mg/dL, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of creatinine in serum and urine.

Specifications

Specifications for Creatinine Reagent Kit (Jaffe Kinetic)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Jaffe kinetic (alkaline picrate)
Sample type	Serum free of haemolysis; 12 h or 24 h urine diluted 1:100 with distilled water
Wavelength	492 nm (480–520 nm)
Reaction type	Fixed time
Reagent volume	1 mL
Sample volume	100 µL
Incubation	Delay 30 s, read 60 s, interval 60 s
Reaction temperature	37 °C
Light path	1 cm
Standard	2.0 mg/dL
Linearity	25 mg/dL
Reagent composition	Reagent 1: alkaline reagent; reagent 2: picric reagent; creatinine standard 2.0 mg/dL
Reference interval	Serum, male: 0.7–1.3 mg/dL; female: 0.5–1.02 mg/dL Reference intervals are indicative; each laboratory should establish its own (see the kit insert).