Chloride Reagent Kit (Mercuric Thiocyanate)

Mercuric thiocyanate reagent for quantitative chloride in serum, urine and cerebrospinal fluid.

Method: Mercuric thiocyanate
Sample: Serum free of haemolysis, urine, cerebrospinal fluid
Linear up to 120 mEq/L
Liquid stable, ready to use pack
Reads at 510 nm

For in vitro diagnostic use by laboratory and healthcare professionals only. Please refer to the instructions for use supplied with each product for complete protocol and performance details.

Overview

For in vitro quantitative determination of chloride concentration in serum, urine and cerebrospinal fluid. Principle: In an acidic environment, chloride reacts with mercuric thiocyanate in the presence of ferric nitrate to form a coloured complex; the colour intensity is proportional to chloride concentration. If chloride exceeds 120 mEq/L, dilute the sample, re-assay and multiply by the dilution factor.

Intended use: For in vitro quantitative determination of chloride concentration in serum, urine and cerebrospinal fluid.

Specifications

Specifications for Chloride Reagent Kit (Mercuric Thiocyanate)
Shelf life	12 months
Storage	2–8 °C, protected from light
Method	Mercuric thiocyanate
Sample type	Serum free of haemolysis, urine, cerebrospinal fluid
Wavelength	510 nm
Reaction type	End point (increasing)
Reagent volume	1 mL
Sample volume	10 µL
Incubation	1 minute
Reaction temperature	37 °C
Light path	1 cm
Standard	100 mEq/L
Linearity	120 mEq/L
Reagent composition	Reagent 1: chloride reagent; chloride standard 100 mEq/L